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Table 2 Segregation patterns of CRISPRCas9-medicated targeted mutagenesis during the T0 to T1 generation.

From: CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance

Mutant plants

T0 generation

Mutation transmission in the T1 generation

Genotype

Mutation type

No. of plants tested

WT

Bi-allele

Homozygote

Heterozygote

Chimeric

Line 16

(T2) Heterozygote

(wt, i1)

21

1

1 (d3, i1), 1 (d2, i1)

9 (i1)

6 (wt, i1)

3

Line 21

(T1) Heterozygote

(wt, i1)

22

0

2 (i1, d4), 1 (s4, i1), 1 (i1, d5),

6 (i1)

11 (wt, i1)

1

(T2) Heterozygote

(wt, s3/d4)

22

2

0

7 (d4)

13 (wt, d4),

0

Line 62

(T1) Biallelic

(i1, d4)

20

0

10 (i1, d4), 1 (i1, d8),

3 (d4), 6 (i1)

0

0

(T2) Heterozygote

(wt, d4)

20

0

2(d3, d4)

5 (d4)

13 (wt, d4)

0

  1. wt wild-type sequence without mutations detected at target sequences, d# the number of bases deleted from the target sequences, i# the number of bases inserted at target sequences, s# the number of bases substituted origin target sequences.
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BMC Plant Biology

ISSN: 1471-2229

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