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Fig. 4 | BMC Plant Biology

Fig. 4

From: Generation of a new thermo-sensitive genic male sterile rice line by targeted mutagenesis of TMS5 gene through CRISPR/Cas9 system

Fig. 4

Pollen fertility and grain set of cv. YK17 and T2 generation tms5–1 and tms5–2 plants grown under various daily average regimes. a [a-f] cv. YK17, [g-l] tms5–1, [m-r] tms5–2 plants exposed to 22 °C, 24 °C or 26 °C. b Appearance of the whole plant of (a) cv. YK17, (b) tms5–1, (c) tms5–2 grown at 22 °C. c-e Grain set at 22 °C, 24 °C or 26 °C. Values are shown as mean ± SD (n = 20). Asterisks (* P < 0.05; ** P < 0.01) indicate statistically significant differences between means of a mutant and cv. YK17, as determined by a student’s t-test. Bars in (A) [a, c, e, g, i, k, m, o, q]: 100 μm; [b, d, f, h, j, l, n, p, r]: 5 cm; in B: 15 cm

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