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Fig. 5 | BMC Plant Biology

Fig. 5

From: Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

Fig. 5

Inheritance and segregation of GmFAD2 small deletions at T1 generation. a Diagram of PCR-based genotyping for small deletions. An example of Indel-specific primer, D22-F, designed for transgenic events ND1–22 and ND1–15 is shown. b PCR-based genotyping of T1 plants from transgenic event ND1–22 showing homozygous mutation in GmFAD2–1B. c PCR-based genotyping of T1 plants from transgenic event ND1–15 showing heterozygous mutation in GmFAD2–1B. PCR-based genotyping results for bar and Cas9 genes are shown in (b) and (c). (+) gene was detected, (−) gene was not detected

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BMC Plant Biology

ISSN: 1471-2229

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