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Table 2 fatty acid composition of tobacco leaf from wild type and fad2–2 mutant plants

From: Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2–2

Vector

Fatty acid composition (mol%)

16:0

18:0

18:1Δ9

18:2Δ9, 12

18:3Δ9, 12, 15

WT

12.6 ± 0.2

4.9 ± 0.1

6.1 ± 0.1

12.2 ± 0.2

64.1 ± 1.7

fad2–2#06

12.7 ± 0.3

4.5 ± 0.2

6.3 ± 0.2

12.7 ± 0.1

63.8 ± 1.2

fad2–2#14

11.9 ± 0.2

5.1 ± 0.3

5.8 ± 0.1

13.1 ± 0.3

64.8 ± 2.1

  1. 16:0, palmitic acid; 18:0, stearic acid; 18:1Δ9, oleic acid; 18:2Δ9, 12, linoleic acid; 18:2Δ9, 12, 15, linolenic acid. Data are means ± SD of three independent experimental replicates
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BMC Plant Biology

ISSN: 1471-2229

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