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Fig. 15 | BMC Plant Biology

Fig. 15

From: Functional identification of BpMYB21 and BpMYB61 transcription factors responding to MeJA and SA in birch triterpenoid synthesis

Fig. 15

Interaction of MBS elements with BpMYB21 and BpMYB61. Binding of BpMYB21 and BpMYB61 to MBS by Y187 was determined. Three to five tandem copies of the MBS sequence were cloned separately into pHIS2 as reporter constructs. Transformants were screened on SD/−His/−Leu/−Trp (TDO) medium with 100 mM 3-amino-1,2,4-triazole (3-AT). p53HIS2/pGADT7-p53 and p53HIS2/pGADT7-BpMYB21 (and p53HIS2/p GADT7-BpMYB61) served as positive and negative controls, respectively. Triangles indicate the yeast dilutions (1:1, 1:10, 1:100 and 1:1000)

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BMC Plant Biology

ISSN: 1471-2229

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