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Table 1 Efficiency of transgenic plant production after biolistic- mediated transformation of immature embryo-derived tissues of emmer wheat ‘Runo’ (T.dicoccum (Schrank))

From: A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gun

Morphogenic tissue typea

Experiment No.

No.

independent explants bombarded

No. independent explants produced plantlets after dual GFP + PPT selection

No. putative transgenic plants selected in vitro

No. independent explants produced transgenic (PCR-positive) events

Transformation

frequency, % b

Total

PCR-positive

for BAR

Type I

1

82

9

10

9

8

9.8

2

83

6

7

6

5

6.0

3

50

3

3

3

3

6.0

4

80

11

12

11

10

12.5

5

58

11

11

11

11

19.0

6

100

18

24

23

17

17.0

7

50

9

9

9

9

18.0

8

150

24

25

23

22

14.7

Total / Mean

 

653

91

101

95

85

12.9

Type II

1

50

0

0

0

0

0.0

2

100

2

2

2

2

2.0

3

48

0

0

0

0

0.0

4

103

2

2

2

2

1.9

5

100

9

11

10

8

8.0

6

75

4

6

6

14

5.3

Total / Mean

 

476

17

21

20

16

3.4

  1. aType I – nodular white morphogenic calli, Type II – nodular translucent morphogenic calli
  2. bcalculated as percentage of the independent bombarded explants produced at least one PCR- positive transgenic plant T0