Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content

Table 5 Efficiency of transgenic plant production after biolistic- mediated transformation of immature embryo-derived tissues of Timopheevi wheat (T.timopheevii Zhuk)

From: A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gun

Callus induction/selection mediuma

No. IE explant cultured

Morphogenic callus formation (%)b

No. bombarded explants

No. explants produced transgenic plants after dual GFP + PPT selection

Transformation efficiency (%)

Daminozide 0 mg/l

845

30.2a

255

0

0.0

Daminozide 12.5 mg/l

652

57.7b

376

2

0.5

Daminozide 25.0 mg/l

538

65.4b

352

0

0.0

  1. aMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l 2,4-D; 3 mg/l PPT was additionally added into selection medium
  2. bMeans having the same letter in the column has no significant differences according to Duncan’s multiple range test (P < 0.05)
Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us