Fig. 4

RT-qPCR analysis of selected genes predicted to be directly targeted by the POLV and JMJ14 proteins during TuMV infection. Direct targets were genes that were induced in mutant polv or jmj14-infected mutants compared to wild-type infected plants at 4 and/or 7 days post-inoculation (dpi) and overlapped with specific epigenetic marks or proteins. POLV predicted targets (NAC003, LURP1-LIKE, MRD1, ASCO, CSA1, RLP43, and PP2-A5) and JMJ14 predicted targets (CNGC19, RPW8, CDC48B, PYL5, and RGL3) were quantified at 4 and 7 dpi (TuMV or mock inoculation). For the relative quantification, A. thaliana endogenous genes SAND and PP2A were used as references. Pairwise t-test comparisons were performed with Bonferroni correction tests; ***P < 0.001; **P < 0.01; *P < 0.05; ns., not significant