Fig. 7

Virus quantification and transcriptome analysis of wild-type A. thaliana plants subjected to multiple virus stresses. a Absolute RT-qPCR analysis of TuMV load in samples that had been mock- or ROS-TuMV-stimulated prior to TuMV challenge. Inoculated leaves were removed 26-28 hours later to prevent virus spread or account for mechanical stress responses. In both conditions, TuMV was inoculated three days after leaf removal. For each condition, six individual plants were sampled four and eight days after the second TuMV inoculation (dpi). b TuMV symptom severity progression in unstimulated (n = 60) and TuMV-stimulated (n = 20) plants. Symptoms were scored daily using the scale described in the Methods section, with higher numbers indicating more severity. c Gene ontology analysis of induced and repressed genes. DESEq2 was used for differential expression analysis, with TuMV-stimulated plants as treatments and mock-stimulated plants as controls. The size of the circles represents the degree of enrichment, whereas the colors of the heat maps represent the adjusted P values (p.adjust)