Fig. 5
Rescue of dar-1 and identification of dar-2 (A) Ectopic expression of MtrunA17_Chr2g0304631 in roots of the dar-1 mutant restores nodule number regulation. The dar-1 plants with the empty vector were significantly different from wild type, while dar-1 plants with the MtrunA17_Chr2g0304631 expression construct were similar to wild type (Kruskal–Wallis test p = 4.799 × 10–06; different letters denote significant differences between samples (p < 0.05) in pairwise comparisons using Dunn’s post-hoc test). For each condition, 10 to 11 plants were analyzed. B A line derived from NF2117 (dar-2) carrying a Tnt1 insertion in MtrunA17_Chr2g0304631 showed an increased number of nodules compared to its parental wild type line, R108. (*, p < 5 × 10–4, Student’s t-test; n = 4 to 23 plants)