Fig. 9

Protein structure of DAR (A) The transmembrane topology of DAR. The predicted structure includes five transmembrane spanning domains. Cytoplasmic links between consecutive TM domains are short, while on the side of the membrane annotated as non-cytoplasmic by Phytozome there are regions of 78 to 185 amino acids. B Displayed is the AlphaFold predicted structure of the DAR protein and corresponding confidence metrics. The left panel displays the predicted 3D structure of the DAR protein with the respective confidence level for each region of the DAR. * indicates the start of the protein. Blue: Very high confidence (pLDDT > 90), Cyan: Confident (90 > pLDDT > 70), Yellow: Low confidence (70 > pLDDT > 50), Orange: Very low confidence (pLDDT < 50). The right panel shows the predicted alignment error (PAE) heatmap that represents the expected position error (in Ångströms) for each pair of residues in the predicted structure, where darker and lighter green indicate the lower and higher expected error rate respectively. The diagonal represents self-alignment (perfect prediction). The color scale at the bottom shows the range of expected position errors