Genome-wide analysis of miR172-mediated response to heavy metal stress in chickpea (Cicer arietinum L.): physiological, biochemical, and molecular insights

Table 1 The variance analysis results of the parameters¹

Parameters	Sources of variation (Mean square)
Parameters	Genotype (G) (df = 1)	Treatment (T) (df = 5)	G x T (df = 5)	Error (df = 36)
SL (cm)	26.255**	12.280**	15.330**	0.196
RL (cm)	31.687**	77.313**	3.225**	0.486
CMD (%)	2.154^ns	264.768**	23.005**	0.756
RWC (%)	33.413**	391.208**	91.922**	0.682
CC (SPAD)	8.841**	62.667**	120.458**	0.060
S-H₂O₂ (µmol/g)	7840.109**	32571.190**	14355.340**	109.038
R-H₂O₂ (µmol/g)	159.547^ns	33539.944**	5560.888**	202.365
S-MDA (µmol/g)	0.283**	0.434**	0.267**	0.001
R-MDA (µmol/g)	0.731**	1.091**	0.261**	0.001
S-SOD (EU/g)	0.232*	2.212**	0.435**	0.035
R-SOD (EU/g)	0.301**	0.964**	0.929**	0.017
S-CAT	0.116**	0.312**	0.024**	0.00011
R-CAT	0.016**	0.194**	0.098**	0.0012
S-POD (EU/g)	271.395**	39.773**	25.099**	0.019
R- POD (EU/g)	167.626**	85.049**	29.842**	0.005
S-APX (EU/g)	0.528**	0.377**	0.125**	0.00003461
R- APX (EU/g)	0.009**	0.277**	0.071**	0.00004168
S-Proline (µmol/g)	0.065**	0.024**	0.033**	0.000
R-Proline (µmol/g FW)	0.031**	0.065**	0.032**	0.000

¹SL Shoot length, RL Root length, CMD Cell membrane damage, RWC Relative water content, CC chlorophyll content, S Shoot, R Root, H₂O₂ Hydrogen peroxide, MDA Malondialdehyde, SOD Superoxide dismutase, CAT Catalase, POD Peroxidase, and APX; Ascorbate peroxidase
*. **: significant at 0.05 and 0.01 level. respectively. ^ns: non-significant at 0.05 level

ISSN: 1471-2229