Fig. 3

The polyadenylation landscape of Arabidopsis lncRNAs. (A) Pie chart showing the numbers and proportions of poly(A) lncRNAs and non-poly(A) lncRNAs. (B) Violin plot exhibiting the poly(A) tail lengths of lncRNAs (blue) and of protein-coding RNAs (red). The poly(A) tails of lncRNAs were slightly shorter than those of protein-coding RNAs (Mann-Whitney U test; ***, p < 0.001). (C) Box plot comparing the transcript lengths of poly(A) lncRNAs (orange) to those of non-poly(A) lncRNAs (purple). Poly(A) lncRNAs possessed significantly longer transcripts than non-poly(A) lncRNAs (Mann-Whitney U test; ***, p < 0.001). (D) Box plot comparing the expression levels between poly(A) lncRNAs (orange) and non-poly(A) lncRNAs (purple). Poly(A) lncRNAs were significantly more abundant than non-poly(A) lncRNAs (Mann-Whitney U test; ***, p < 0.001). (E) Scatter plot with linear regression demonstrating a moderately positive correlation between poly(A) tail lengths and transcript lengths of poly(A) lncRNAs (Spearman’s ρ = 0.30, p < 0.001). (F) Scatter plot with linear regression presenting a slightly negative correlation between poly(A) tail lengths and expression levels of poly(A) lncRNAs (Spearman’s ρ = -0.27, p < 0.001)