Foliar application of 24-epibrassinolide enhances leaf nicotine content under low temperature conditions during the mature stage of flue-cured tobacco by regulating cold stress tolerance

Table 5 Foliar application of different EBR compounds significantly improves chemical composition of cured tobacco leaves

Treatment	Total sugar /%	Reducing sugar /%	Total nitrogen /%	Nicotine/%	Potassium/%	Chlorine/%
C3F
CK2	28.36 ± 0.14c	21.43 ± 0.07c	1.60 ± 0.01c	1.74 ± 0.02c	0.87 ± 0.02c	0.50 ± 0.01a
B1	28.46 ± 0.30c	24.11 ± 0.26b	1.75 ± 0.01b	1.97 ± 0.02b	1.04 ± 0.03b	0.42 ± 0.01b
B2	29.36 ± 0.18b	25.14 ± 0.22a	1.80 ± 0.01a	2.03 ± 0.06b	1.33 ± 0.01a	0.43 ± 0.01b
B3	30.24 ± 0.14a	25.31 ± 0.26a	1.82 ± 0.01a	2.16 ± 0.00a	1.38 ± 0.05a	0.42 ± 0.02b
B2F
CK2	24.26 ± 0.14c	18.17 ± 0.05c	1.75 ± 0.01d	2.31 ± 0.04d	0.77 ± 0.01d	0.55 ± 0.01a
B1	25.54 ± 0.24b	19.69 ± 0.66b	1.99 ± 0.04c	2.66 ± 0.02c	0.95 ± 0.01c	0.42 ± 0.00c
B2	27.28 ± 0.41a	20.69 ± 0.08ab	2.17 ± 0.02b	2.80 ± 0.02b	1.14 ± 0.02b	0.44 ± 0.02bc
B3	27.51 ± 0.07a	21.23 ± 0.32a	2.26 ± 0.04a	2.98 ± 0.02a	1.23 ± 0.04a	0.45 ± 0.00b

Note C3F and B2F indicate as shown in Table 2. CK2, B1, B2 and B3 indicate as shown in Table 3. The data presented are the mean ± SD. Different letters indicate significant differences among the treatments based on Duncan’s test (P < 0.05)

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