Fig. 9

Prediction of upstream transcription factors and interacting proteins for four differentially expressed genes, and the expression levels of different genes in the transcriptome under drought and cadmium stress. A Venn diagram of different genes in roots under cadmium and drought stress. The two gene sets are filled with different colors, and the overlapping area represents the genes shared between the gene sets, with numbers indicating the count of shared genes. B, C Prediction of upstream transcription factors and interacting proteins for the target genes. Nodes represent gene or protein names, with pink nodes indicating transcription factors. The size of the nodes represents gene connectivity, with larger nodes indicating stronger connectivity. Lines connecting nodes represent the interaction relationships between genes or proteins. The color of the lines (min p-value) represents the probability of motif occurrence or protein interaction, with smaller p-values indicating more reliable results. The thickness of the lines (max score) represents the motif occurrence score, with thicker lines indicating a higher possibility of transcription factor binding to the output sequence or stronger protein interaction. D A heatmap shows the expression profiles of six predicted transcription factors in roots under drought stress. E A heatmap shows the expression profiles of six predicted transcription factors in roots under cadmium stress. F A heatmap shows the expression profiles of four predicted interacting proteins in roots under drought stress. G A heatmap shows the expression profiles of four predicted interacting proteins in roots under cadmium stress