Fig. 2

Metabolite content detection in DaERF9 overexpressing. (a) Expression level of DaERF9 in DaERF9 overexpressing plants. (b) Differences in terpenoid metabolites in DaERF9 overexpressing plants. (c) Clustering analysis of terpenoid metabolites in DaERF9 overexpressing plants. The heat map represents metabolites whose abundance is up-regulated, and blue represents metabolites whose abundance is down-regulated. The red letters are triterpenoids. (d) VIP bar chart of terpenoid components. The heat map represents metabolites whose abundance is up-regulated, and blue represents metabolites whose abundance is down-regulated. The red letters are triterpenoids. (e) Content of asperosaponin VI synthesis precursor compounds. Red font metabolites are triterpenoid components. Data represent the values of the mean ± SE (standard error) of three replicates. Note Different lowercase letters indicate significant differences between treatments under the same indicator (p < 0.05)