Fig. 1

ABA induced RAE1 gene expression in Arabidopsis. (A) Cis regulatory elements in RAE1 promoter (minus 2.6 kb) were analyzed by PlantCARE. ABRE and MBS elements in RAE1 promoter are highlighted by colored arrows. (B) The relative expression of RAE1 in 7 d old plate grown Arabidopsis seedlings in response to ABA treatment are shown as column graphs. The level of RAE1 transcripts after a time course of 10 µM ABA treatments (0, 1.5, 3, 4.5 h) were determined by qRT-PCR. (C) GUS-stained root tips from 5 d old seedlings of an Arabidopsis line with stable expression of pRAE1: GUS were treated with or without 10 µM ABA treatment for 3 h for short-term ABA response analysis (scale bar = 100 μm). (D) GUS activity was determined in 2 d old seedlings of the pRAE1: GUS line with or without 10 µM ABA treatment. (E) GUS stained 2 to 6 d old seedlings of the pRAE1: GUS line with or without 1 µM ABA treatment from seeds (scale bar = 200 μm). Error bars represent the standard deviation for four biological replicates for qRT-PCR and GUS activity assay (* indicates significant difference (P < 0.05), ** indicates highly significant difference (P < 0.01), as per Student’s t-test)