Fig. 4

Alterations in gene expression of ABA-associated transcription factors and regulatory genes in rae1-b. (A) Principal component analysis (PCA) was conducted using the transcript levels of all measured genes in wild type (WT) and rae1-b. PC1 and PC2 values are represented on the x and y axes, respectively. RNA-seq was performed using total RNA extracted from seven-day-old Arabidopsis seedlings treated with 10 µM ABA for three hours (DataS1). (B) Heatmap displaying transcription factors with significant expression changes (fold change > 1.5 or < − 1.5, P < 0.05) in rae1-b compared to WT (DataS2). (C) Heatmap illustrating significant expression changes in ABA response genes, including downstream targets of ABI5 (MFT, EM6, RD29B, and RAB18) and PP2C family members (ABI1, ABI2) in rae1-b. The expression level of ABI5, ABI5 E3 ligase and proteins associated with ABI5 degradation (MIEL1, ABD1, DDB1B, RACK1B/C), are also shown. Genes which were characterized as ABA positive regulators/ABI5 targets were colored in orange fonts, negative regulators were colored in blue fonts