Fig. 6

Differential sensitivity to exogenous ABA treatment in rae1-b and stop1-2 mutants indicated by varied MFT expression. (A) Seeds of rae1-b, stop1-2, and wild type were cultivated on half-strength MS medium with or without 1 µM ABA treatment. Representative images of 9-day-old seedlings are shown. (B) The proportion of 9 d old seedlings with cotyledon greening are shown as column graphs. Grouping is determined by one-way ANOVA (P < 0.05, Duncan’s multiple-range test). Error bars represent the standard deviation across three representative plates. (C-I) Seven-day-old Arabidopsis seedlings (WT, rae1-b, stop1-2, and STOP1OE2) grown on plates were transferred to new plates with or without 10 µM ABA and incubated for three hours. Gene expression of ABI5, ABI1, ABI2, MFT, EM6, RD29B, and RAB18 was quantified by qRT-PCR. Relative expression levels, normalized to WT under control conditions, are shown as column graphs. Error bars represent the standard deviation for biological triplicates. Grouping is determined by a Student’s t-test (P < 0.05)