Beyond nitrate transport: AtNRT2.4 responds to local and systemic nitrogen signaling in Arabidopsis

BMC Plant Biology

Table 1 AtNRT2.4 responds to local N-deficiency and systemic nitrate signals

	Local N-deficiency signal (C.NaCl/C.NaNO₃)			Systemic N-sufficiency signal (Sp.NaCl/C.NaCl)			Systemic N-deficiency signal (Sp.NaNO₃/C.NaNO₃)
	Col-0	nrt2.4	D-value	Col-0	nrt2.4	D-value	Col-0	nrt2.4	D-value
Total root length	1.47 ± 0.09	−0.24 ± 0.12	1.71 ± 0.09↑	−1.66 ± 0.14	−1.20 ± 0.04	−0.46 ± 0.14	1.47 ± 0.12	−0.33 ± 0.01	1.80 ± 0.12↑
Number of lateral root	1.04 ± 0.10	−0.53 ± 0.19	1.57 ± 0.10↑	−1.86 ± 0.15	−0.70 ± 0.11	−1.16 ± 0.15↓	1.26 ± 0.10	−0.55 ± 0.10	1.81 ± 0.10↑
AtNRT2.4	2.42 ± 0.02	0.07 ± 0.10	2.35 ± 0.02↑	−3.51 ± 0.12	−2.43 ± 0.07	−1.08 ± 0.12↓	−2.24 ± 0.13	−3.69 ± 0.09	1.44 ± 0.13↑
AtANR1	−0.05 ± 0.06	−3.77 ± 0.09	3.72 ± 0.06↑	−0.31 ± 0.03	3.56 ± 0.04	−3.87 ± 0.03↓	2.39 ± 0.03	0.43 ± 0.02	1.96 ± 0.03↑
AtCIPK23	1.61 ± 0.06	−0.43 ± 0.07	2.04 ± 0.06↑	−1.43 ± 0.04	−0.62 ± 0.07	−0.81 ± 0.04	0.66 ± 0.05	−0.74 ± 0.05	1.40 ± 0.05↑
AtNRT2.5	1.42 ± 0.09	0.43 ± 0.21	0.99 ± 0.09	−3.25 ± 0.35	−2.24 ± 0.18	−1.00 ± 0.35	−3.71 ± 0.30	−3.22 ± 0.18	−0.48 ± 0.30
AtCEP3	1.31 ± 0.06	−0.22 ± 0.07	1.53 ± 0.06↑	−2.79 ± 0.15	−1.79 ± 0.09	−1.00 ± 0.15	−1.45 ± 0.15	−1.88 ± 0.06	0.43 ± 0.15
AtTCP20	1.02 ± 0.06	−0.09 ± 0.05	1.11 ± 0.06↑	−1.45 ± 0.10	−0.18 ± 0.04	−1.27 ± 0.10↓	−0.43 ± 0.05	−0.50 ± 0.06	0.06 ± 0.05
AtCBL1	1.30 ± 0.06	−0.22 ± 0.05	1.52 ± 0.06↑	−2.74 ± 0.09	−1.75 ± 0.04	−0.99 ± 0.09	−1.38 ± 0.08	−1.87 ± 0.06	0.49 ± 0.08

1 The ratios of total root length, lateral root number, and gene expression levels between the treatments C.NaCl/C.NaNO₃, Sp.NaCl/C.NaCl and Sp.NaNO₃/C.NaNO₃, were calculated as'a'. The values in Col-0 and nrt2.4 columns were calculated with the logarithm base 2 transformations of'a'(log2(a)), mean ± SE, n = 4
2 The"D-value"column in the table indicates the difference between Col-0 and nrt2.4, the calculation formula is:"D-value = log2_(Col-0)—log2_(nrt2.4)". When the value in Col-0, nrt2.4 or D-value column is greater than 1 or less than −1, it indicates a significant effect. Conversely, a value within the range of (−1, 1) suggests no significant effect. The direction of the arrows is determined by the D-value. Specifically, upward arrows indicate the promoting impact of AtNRT2.4 on root development or gene expression, whereas downward arrows denote the inhibitory effect

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